551 Combining Laser Capture Microdissection and RNAseq Analysis to Generate a Human Anagen Hair Follicle Transcriptome Atlas

While this is available for murine HFs (www.hair-gel.net), systematic characterization of the transcriptome specific human hair follicle (HF) compartments remains a major unmet challenge in research. Here, we have aimed to initiate generation such an atlas reveal HF compartment-specific transcriptome. We performed laser capture microdissection (LCM) on 8 selected compartments, i.e. bulge outer root sheath (ORS), suprabulbar ORS, inner (IRS), precortical matrix (HM), germinative HM, dermal papilla (DP), lower cup (DC), and connective tissue (CTS), anagen VI scalp HFs, analysed these by RNAseq. Principal component analysis (PCA) was used validate distinctness microdissected compartments. Biological replicates were clearly separated high degree similarity distinct transcriptional profiles each examined compartment seen. Afterwards, matched well-known signature genes correct For example, VCAN (versican) ALPL (alkaline phosphatase) correctly expressed DP, DC, CTS, while TCHH (trichohyalin) solely found proximal IRS, KRT85 (Keratin 85) KRT31 31) almost exclusively HM. These transcriptomic data generate easily accessible database identifying defining gene expression profiles, which can be complemented over time ultimately made publicly accessible. This map associated interactive will support identification genes, facilitates development compartment-specific, therapeutic interventions management disorders.